准教授　　　　　原田　義規

助教（学内講師）小形　岳寛
助教　　　　　　中尾　龍太

助教　　　　　　望月　健太郎

 

特任教授　　　　田中　秀央

特任教授　　　　髙松　哲郎

バイオフォトニクス（最新の光技術とバイオテクノロジーの融合）による、機能分子のイメージングと機能制御技術を用いて、病気の発生メカニズム解明を行っている。独自に開発した様々な先端光学顕微鏡を駆使し、病態における機能的・形態的変化を分子・細胞・組織・臓器レベルで、同時にビジュアルイメージとして捉えることにより、病態の発生機序解明と革新的な組織診断技術の開発を目指す。

 

研究内容

1. 心筋機能分子の可視化による心臓の病態の解明
2. 多光子励起による細胞操作
3. ラマン散乱分光法を用いた心筋バイアビリティーに関する研究
4. 光学的手法を用いた腫瘍の微小環境に関する研究
5. 微小癌やリンパ節転移の検出を目指した分子イメージング
6. プロトポルフィリンによる癌の蛍光検出とヘム生合成調節機序に関する研究

1. Helal K.M, Cahyadi H, Taylor J.N, Okajima A, Tabata K, Kumamoto Y, Mochizuki K,Itoh Y, Takamatsu T, Tanaka H, Fujita K, Komatsuzaki T, Harada Y. Raman imaging of rat non-alcoholic fatty liver tissues reveals distinct biomolecular states FEBS Lett. doi:10.1002/1873-3468.14600 (2023)

 

2. Morishita Y, Tamura S, Mochizuki K, Harada Y, Takamatsu T, Hosoi H, Tanaka H.Generation of myocyte agonal Ca2+ waves and contraction bands in perfused rat hearts following irreversible membrane permeabilisation Sci. Rep. 13, 803 (2023)

 

3. Hayakawa M, Taylor J.N, Nakao R, Mochizuki K, Sawai Y, Hashimoto K, Tabata K,Kumamoto Y, Fujita K, Konishi E, Hirano S, Tanaka H, Komatsuzaki T, Harada Y.Lipid droplet accumulation and adipophilin expression in follicular thyroid carcinoma Biochem. Biophys. Res. Commun. 640, 192-201 (2023)

 

4. Harada Y, Sogame Y, Nakao R, Ogata T, Yasuda H, Sakagami J, Itoh Y, Tanaka H.Autopsy of a Patient with Primary Pancreatic Lymphoma with Findings Resembling Severe Acute Pancreatitis Int. Med. 0757-22 (2022)

 

5. Harada Y, Murayama Y, Takamatsu T, Otsuji E, Tanaka H Fluorescence Imaging for Tumor Detection: Recent Advances and Challenges Int. J. Mol. Sci. 23, 6478 (2022)

 

6. Takemura M, Mochizuki K, Harada Y, Okajima A, Hayakawa M, Dai P, Itoh Y, Tanaka H. Label-free Assessment of the Nascent State of Rat Non-alcoholic Fatty Liver Disease Using Spontaneous Raman Microscopy Acta Histochem. Cytochem. 55, 57-66(2022)

 

7. Beika M, Harada Y, Minamikawa T, Yamaoka Y, Koizumi N, Murayama Y, Konishi H,Shiozaki A, Fujiwara H, Otsuji E, Takamatsu T, Tanaka H. Accumulation of Uroporphyrin I in Necrotic Tissues of Squamous Cell Carcinoma after Administration of 5-Aminolevulinic Acid Int. J. Mol. Sci. 22, 10121 (2021)

 

8. Ikemoto K, Hashimoto K, Harada Y, Kumamoto Y, Hayakawa M, Mochizuki K, Matsuo K,Yashiro K, Yaku H, Takamatsu T, Tanaka H. Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts Acta Histochem.Cytochem. 54, 65-72 (2021)

 

9. Morita M, Hideo T, Kumamoto Y, Nakamura A, Harada Y, Ogata T, Sakaguchi K,Taguchi T, Takamatsu T. Fluorescence-based discrimination of breast cancer cells by direct exposure to 5-aminolevulinic acid Cancer Med.

8, 5524-5533 (2019)

 

10. Helal KM, Taylor JN, Cahyadi H, Okajima A, Tabata K, Itoh Y, Tanaka H, Fujita K,Harada Y, Komatsuzaki T. Raman spectroscopic histology using machine learning for nonalcoholic fatty liver disease FEBS Lett. 593, 2535-2544 (2019)

 

11. Taylor JN, Mochizuki K, Hashimoto K, Kumamoto Y, Harada Y, Fujita K, Komatsuzaki T. High-resolution Raman microscopic detection of follicular thyroid cancer cells with unsupervised machine learning J. Phys. Chem. B 123, 4358-4372 (2019)

 

12. Yamamoto T, Minamikawa T, Harada Y, Yamaoka Y, Tanaka H, Yaku H, Takamatsu T. Label-free evaluation of myocardial infarct in surgically excised ventricular myocardium by Raman spectroscopy Sci. Rep. 8, 14671 (2018)

 

13. Matsuyama T, Tanaka H, Ishibashi-Ueda H, Takamatsu T. Spatiotemporally non-uniform Ca 2+ dynamics of cardiac Purkinje fibers in mouse myocardial infarct J. Histochem. Cytochem. 65, 655-667 (2017)

 

14. Tanaka H, Matsuyama T, Takamatsu T. Towards an integrated understanding of cardiac arrhythmogenesis - Growing roles of experimental pathology Pathol. Int. 67, 8-16 (2017)

 

15. Ohira S, Tanaka H, Harada Y, Minamikawa T, Kumamoto Y, Matoba S, Yaku H, Takamatsu T. Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy Sci. Rep. 7, 42401 (2017)

 

16. Koizumi N, Harada Y, Beika M, Minamikawa T, Yamaoka Y, Dai P, Murayama Y, Yanagisawa A, Otsuji E, Tanaka H, Takamatsu T. Highly sensitive fluorescence detection of metastatic lymph　nodes of gastric cancer with photo-oxidation of protoporphyrin IX Eur. J. Surg. Oncol. 42, 1236-1246 (2016)

 

17. Nishiki-Muranishi N, Harada Y, Minamikawa T, Yamaoka Y, Dai P, Yaku H, Takamatsu T. Label-free evaluation of myocardinal infarction and its repair by spontaneous Raman spectroscopy Anal. Chem. 86, 6903-10 (2014)

 

18. Matsuyama T, Tanaka H, Adachi T, Jiang Y, Ishibashi-Ueda H, Takamatsu T. Intrinsic left atrial histoanatomy as the basis for reentrant excitation causing atrial fibrillation/flutter in rats Heart Rhythm 10, 1342-8 (2013)

 

19. Imaizumi K, Harada Y, Wakabayashi N, Yamaoka Y, Konishi H, Dai P, Tanaka H,Takamatsu T. Dual-wavelength excitation of mucosal autofluorescence for precise detection of diminutive colonic adenomas Gastrointest. Endosc. 75, 110-7 (2012)

 

20. Fujiwara K, Tanaka H, Mani H, Nakagami T, Takamatsu T. Burst Emergence of Intracellular Ca2+ Waves Evokes Arrhythmogenic Oscillatory Depolarization via the Na+-Ca2+ Exchanger Simultaneous Confocal Recording of Membrane Potential and Intracellular Ca2+ in the Heart. Circ Res 103, 509-18 (2008).

 

21. Hamamoto T, Tanaka H, Mani H, Tanabe T, Fujiwara K, Nakagami T, Horie M, Oyamada M, Takamatsu T. In situ Ca2+ dynamics of Purkinje fibers and its interconnection with subjacent ventricular myocytes. J Mol Cell Cardiol 38, 561-9 (2005).

 

22. Kaneko T, Tanaka H, Oyamada M, Kawata S, Takamatsu T. Three distinct types of Ca2+ waves in Langendorff-perfused rat heart revealed by real-time confocal microscopy. Circ Res 86, 1093-1099 (2000).

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