細胞分子機能病理学/病理学教室(細胞分子機能病理学部門)

細胞分子機能病理学HP http://www.f.kpu-m.ac.jp/k/pcr/

大学院科目名 細胞分子機能病理学
医学部教室名 病理学教室(細胞分子機能病理学部門)
スタッフ
 教授  田中 秀央
 
 
准教授     原田 義規
講師(学内)小形 岳寛
助教    熊本 康昭
助教    中尾 龍太
研究内容
バイオフォトニクス(最新の光技術とバイオテクノロジーの融合)による、機能分子のイメージングと機能制御技術を用いて、病気の発生メカニズム解明を行っている。独自に開発した様々な先端光学顕微鏡を駆使し、病態における機能的・形態的変化を分子・細胞・組織・臓器レベルで、同時にビジュアルイメージとして捉えることにより、病態の発生機序解明と革新的な組織診断技術の開発を目指す。
 
研究内容
1. 心筋機能分子の可視化による心臓の病態の解明
2. 多光子励起による細胞操作
3. ラマン散乱分光法を用いた心筋バイアビリティーに関する研究
4. 光学的手法を用いた腫瘍の微小環境に関する研究
5. 微小癌やリンパ節転移の検出を目指した分子イメージング
6. プロトポルフィリンによる癌の蛍光検出とヘム生合成調節機序に関する研究
研究業績

1.     Spatiotemporally non-uniform Ca2+ dynamics of cardiac Purkinje fibers in mouse myocardial infarct. J Histochem Cytochem 65, 2017 PMID: 28903013.

2.     Efficient fluorescence detection of protoporphyrin IX in mesenteric lymph nodes of murine colorectal cancer stained with indigo carmine. Photodiag Photodyn Ther 19, 175-180, 2017.

3.     Label-free detection of myocardial ischaemia in the perfused rat hearts by spontaneous Raman spectroscopy. Sci Rep 7, 42401, 2017.

4.     Rapid and accurate perioheral nerve imaging by multipoint Raman spectroscopy. Sci Rep 7, 845, 2017.

5.     Towards and integrated understanding of carciac arrhythmogenesis. – Growing roles of experimental pathology. Pathol Int 67, 8 – 16, 2017.

6.     Histopathological characteristics of post-inflamed coronary arteries in Kawasaki disease-like vasculitis of rabbits. Acta Histochem Cytochem 49, 29-36, 2016.

7.     Highly sensitive fluorescence detection of metastatic lymph nodes of gastric cancer with photo-oxidation of protoporphyrin IX. Eur J Surg Oncol 42 1236 – 1246, 2016.

8.     Simplified and optimized multispectral imaging for 5-ALA-bbased flurescence diagnosis of malignant lesions. Sci Rep 6, 25530, 2016.

9.     How does the Ca2+-paradox Injury induce contracture in the heart? - A combined study of the intracellular Ca2+ dynamics and cell structures in perfused rat hearts - Acta Histochem Cytochem 48: 1-8, 2015.

10.    In vivo detection of rat colorectal cancers by using a dual-wavelength excitation method. Acta Histochem Cytochem. 47:247-54, 2014

11.   Label-free evaluation of myocardinal infarction and its repair by spontaneous Raman spectroscopy. Anal Chem. 86: 6903-10, 2014.

12.   Pacing-induced non-uniform Ca²⁺dynamics in rat atria revealed by rapid-scanning confocal microscopy. Acta Histochem Cytochem.47: 59-65. 2014.

13.   Neutrophil Phagocytosis of Platelets in the Early Phase of 2,4,6-trinitro-1-chlorobenzene (TNCB)-induced Dermatitis in Mice. Acta Histochem Cytochem.47: 67-74. 2014

14.   Detection of lymph node metastases in human colorectal cancer by using 5-aminolevulinic acid-induced protoporphyrin IX fluorescence with spectral unmixing. Int J Mol Sci14: 23140-23152, 2013.

15.   Label-free detection of peripheral nerve tissues against adjacent tissues by spontaneous Raman microspectroscopy. Histochem Cell Biol.139:181-193,2013.

16.   Precise analysis of the autofluorescence characteristics of rat colon under UVA and violet light excitation. Curr Pharm Biotech 14 172-179, 2013

17.   Intrinsic left atrial histoanatomy as the basis for reentrant excitation causing atrial fibrillation/flutter in rats. Heart Rhythm. 10:1342-1348, 2013.

18.   Detection of metastatic lymph nodes using 5-aminolevulinic acid in patients with gastric cancer. Ann Surg Oncol.20: 3541-3548, 2013

19.   Dual-wavelength excitation of mucosal autofluorescence for precisedetection of diminutive colonic adenomas. Gastrointest Endosc. 75:110-7, 2012

20.   Precise detection of lymph node metastases in mouse rectal cancer by useing 5-aminolevulinic acid Int J Cancer. 125:2256-63, 2010.

21.   Intracellular Dynamics of topoisomerase l inhibitor, CPT-11, by slit-scanning confocal Raman microscopy.Histochem Cell Biol.132: 39-46, 2009.

22.   Label-free biochemical imaging of heart tissue with high-speed spontaneous Raman microscopy. Biochem Biophys Res Commun. 382: 370-374, 2009.

23.   Burst Emergence of Intracellular Ca2+ Waves Evokes Arrhythmogenic Oscillatory Depolarization via the Na+-Ca2+ Circ Res. 103: 509-18, 2008.
24.   Generation of reentrant arrhythmias by dominant-negative inhibition of connexin43 in rat cultured myocyte monolayers. Cardiovasc Res 79: 70-79, 2008.

25.   In situ Ca2+ dynamics of Purkinje fibers and its interconnection with subjacent ventricular myocytes. J Mol Cell Cardiol. 38: 561-9, 2005.
26.   In situ visualization of the intracellular Ca2+ dynamics at the border of the acute myocardial infarct Mol Cell Biochem. 248:135-139, 2003.

27.   Excitation-dependent intracellular Ca2+ waves at the border zone of the cryo-injured rat heart revealed by real-time confocal microscopy.J Mol Cell Cardiol. 34:1501-1512, 2002.

28.   Three distinct types of Ca2+ waves in Langendorff-perfused rat heart revealed by real-time confocal microscopy. Circ Res 86:1093-1099, 2000.

 

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