京都府立医科大学：Department of Pathology and Applied Neurobiology

Subject	Department of Pathology and Applied Neurobiology
Staff	Professor ：Aya Miyagawa Lecturer: Takahiro Fujimoto Assistant Professor: Takeshi Yaoi, So Tando
Research Contents	We, the Department of Pathology and Applied Neurobiology, would like to have freshmen to join our laboratory and to work with us to understand the pathology of nervous system. We are involved in teaching basic pathology to the undergraduate students, whom we further provide with opportunities of studying autopsy cases including participation in clinico-pathological conferences as well as of publishing papers as co-authors in the international journals. In the field of basic research, we have been studying pathogenesis of developmental brain disorders employing various methodologies including molecular biology, cell biology, biochemistry, histology, behavioral assays, and imaging. These researches have so far demonstrated the novel effects of an environmental chemical on brain development and the novel functional significance of genetic factors including cell adhesion molecules and synaptic proteins during cortical development. The publications on our research are listed below. The structures as well as functions of human brain are complex and more efforts are needed to disclose the details even in the genomic era. By gaining more insight into the pathogenesis of developmental brain disorders, we are hoping to find ways which might be applicable for clinical treatment and/or prevention of human diseases. Major research projects are as follows: Molecular neurobiological as well as neuropathological studies on the pathogenesis of developmental brain disorders of humans, employing neural stem cells derived from the brains with disorders. Molecular neurobiological studies on the neuronal type of dystrophin, Dp71. Molecular neuropathological studies on the animal models of human neurological disorders, including hereditary microcephaly type 5 (ASPM gene related microcephaly). Molecular neuropathological effects of environmental factors such as endocrine disrupting chemicals and alcohol on developing brains. Molecular and neuropathological studies on neurodegenerative disease, with special references to neurons and glia. Development of novel methodology and apparatus for medical research by application of innovative technology derived from different fields Written by Kyoko Itoh
Achievements	Pooh RK, Machida M, Nakamura T, Uenishi K, Chiyo H, Itoh K, Yoshimatsu J, Ueda H, Ogo K, Chaemsaithong P, Poon LC. Maldevelopment of Sylvian fissure as an early sonographic sign of malformations of cortical development. Ultrasound Obstet Gynecol. 2018 Oct 31. doi: 10.1002/uog.20171. [Epub ahead of print] Minami M, Ikoma K, Horii M, Sukenari T, Onishi O, Fujiwara H, Ogi H, Itoh K, Kubo T. Usefulness of Sweep Imaging With Fourier Transform for Evaluation of Cortical Bone in Diabetic Rats. J Magn Reson Imaging. 2018; 48(2): 389-397. Ogi H, Nitta N, Tando S, Fujimori A, Aoki I, Fushiki S, Itoh K. Longitudinal Diffusion Tensor Imaging Revealed Nerve Fiber Alterations in Aspm Mutated Microcephaly Model Mice. Neuroscience. 2018; 371: 325-336. Tando S, Nagao T, Kayano K, Fushiki S, Itoh K. High-grade transformation/ dedifferentiation of an adenoid cystic carcinoma of the minor salivary gland to myoepithelial carcinoma. Pathol Int. 2018; 68(2): 133-138. Goto S, Ogi H, Fushiki S, Itoh K. Prenatal and lactational bisphenol A exposure does not alter serotonergic neurons morphologically in the murine dorsal raphe nucleus. Brain Dev. 2017; 39(6): 475-482. Fujimoto T, Yaoi T, Fushiki S, Itoh K. Dp71 is regulated by phosphorylation and ubiquitin-proteasome system in neuronal cells. Biochem Biophys Res Commun. 2017; 492(3): 349-355. Toba S, Jin M, Yamada M, Kumamoto K, Matsumoto S, Yasunaga T, Fukunaga Y, Miyazawa A, Fujita S, Itoh K, Fushiki S, Kojima H, Wanibuchi H, Arai Y, Nagai T, Hirotsune S. Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport. Sci Rep. 2017 ; 7(1): 16386. Nishida A, Yasuno S, Takeuchi A, Awano H, Lee T, Niba ET, Fujimoto T, Itoh K, Takeshima Y, Nishio H, Matsuo M. HEK293 cells express dystrophin Dp71 with nucleus-specific localization of Dp71ab. Histochem Cell Biol. 2016; 146(3): 301-309. Tada M, Konno T, Tada M, Tezuka T, Miura T, Mezaki N, Okazaki K, Arakawa M, Itoh K, Yamamoto T, Yokoo H, Yoshikura N, Ishihara K, Horie M, Takebayashi H, Toyoshima Y, Naito M, Onodera O, Nishizawa M, Takahashi H, Ikeuchi T, Kakita A. Characteristic microglial features in patients with hereditary diffuse leukoencephalopathy with spheroids. Ann Neurol. 2016; 80(4): 554-565. Ogi H, Itoh K, Ikegaya H, Fushiki S. Alterations of neurotransmitter norepinephrine and gamma-aminobutyric acid correlate with murine behavioral perturbations related to bisphenol A exposure. Brain Dev. 2015; 37: 739-746. Takeda K, Sowa Y, Nishino K, Itoh K, Fushiki S. Adipose-derived stem cells promote proliferation, migration, and tube formation of lymphatic endothelial cells in vitro by secreting lymphangiogenic factors. Ann Plast Surg. 2015; 74(6): 728-736. Itoh K, Yagita K, Nozaki T, Katano H, Hasegawa H, Matsuo K, Hosokawa Y, Tando S, Fushiki S.An autopsy case of Balamuthia mandrillaris amoebic encephalitis, a rare emerging infectious disease, with a brief review of the cases reported in Japan. Neuropathology. 2015; 35(1): 64-69. Itoh K, Fushiki S. The role of L1cam in murine corticogenesis, and the pathogenesis of hydrocephalus. Pathol Int. 2015; 65(2): 58-66. Tonosaki M, Itoh K, Umekage M, Kishimoto T, Yaoi T, Lemmon VP, Fushiki S. L1cam is crucial for cell locomotion and terminal translocation of the Soma in radial migration during murine corticogenesis. PLoS One. 2014; 9(1): e86186. Fujimoto T, Itoh K, Yaoi T, Fushiki S. Somatodendritic and excitatory postsynaptic distribution of neuron-type dystrophin isoform, Dp40, in hippocampal neurons. Biochem Biophys Res Commun. 2014; 452(1): 79-84. Tando S, Itoh K, Yaoi T, Ogi H, Goto S, Mori M, Fushiki S. Bisphenol A exposure disrupts the development of the locus coeruleus-noradrenergic system in mice. Neuropathology. 2014; 34(6): 527-534. Ogi H, Itoh K, Fushiki S. Social behavior is perturbed in mice after exposure to bisphenol A: a novel assessment employing an IntelliCage. Brain Behav. 2013; 3(3): 223-228. Kishimoto T, Itoh K, Umekage M, Tonosaki M, Yaoi T, Fukui K, Lemmon VP, Fushiki S. Downregulation of L1 perturbs neuronal migration and alters the expression of transcription factors in murine neocortex. J Neurosci Res. 2013; 91(1): 42-50. Itoh K, Yaoi T, Fushiki S. Bisphenol A, an endocrine-disrupting chemical, and brain development. Neuropathology. 2012; 32(4): 447-457. Tozawa T, Itoh K, Yaoi T, Tando S, Umekage M, Dai H, Hosoi H, Fushiki S. The shortest isoform of dystrophin (Dp40) interacts with a group of presynaptic proteins to form a presumptive novel complex in the mouse brain. Mol Neurobiol. 2012; 45(2): 287-297.
Contact	tel 81-75-251-5769 81-75-251-5849 fax 81-75-251-5769 81-75-251-5849 e-mail kxi14(at)koto.kpu-m.ac.jp (please change (at) to @)
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