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Pathology and Cell Regulation

Subject Pathology and Cell Regulation
Professor Hideo Tanaka
Associate Professor Yoshinori Harada
Lecturer Takehiro Ogata
Assistant Professor Ryuta Nakao
Assistant Professor Akihiro Nakamura
Assistant Professor Kentaro Mochizuki
Research Contents
  1. Visualization of dynamics of functional molecules in the heart for understanding mechanisms of heart diseases especially arrhythmias.
  2. Functional inactivation of target proteins by using non-linear optics, e.g., MP-CALI
  3. Elucidation of cellular functions by using advanced optics technologies
  4. Application of autofluorescence imaging for diagnosis of tumors
  5. Molecular imaging of living tissues (e.g., myocardial infarct) by Raman scattering light
  6. Detection of cancers by using 5-ALA, e.g., lymph-node metastasis and extension in tissues

1. Fluorescence-based discrimination of breast cancer cells by direct exposure to 5-aminolevulinic acid. Cancer Med (2019).

2. Raman spectroscopic histology using machine learning for nonalcoholic fatty liver disease. FEBS Lett (2019).

3. Label-free evaluation of myocardial infarct in surgically excised ventricular
myocardium by Raman spectroscopy. Sci Rep 8, 14671 (2018).

4. Spatiotemporally non-uniform Ca2+ dynamics of cardiac Purkinje fibers in
mouse myocardial infarct. J Histochem Cytochem. 65, 655-667 (2017).

5. Towards an integrated understanding of cardiac arrhythmogenesis - Growing roles of experimental pathology. Pathol. Int 67, 8-16 (2017).

6. Label-free detection of myocardial ischaemia in the perfused rat heart by
spontaneous Raman spectroscopy. Sci Rep 7, 42401 (2017).

7. Label-free evaluation of myocardial infarction and its repair by spontaneous Raman spectroscopy. Anal Chem 86, 6903-10 (2014).

8. Intrinsic left atrial histoanatomy as the basis for reentrant excitation causing atrial fibrillation/flutter in rats. Heart Rhythm 10, 1342-8 (2013).

9. Dual-wavelength excitation of mucosal autofluorescence for precise detection of diminutive colonic adenomas. Gastrointest Endosc 75, 110-7 (2012).

10. Precise detection of lymph node metastases in mouse rectal cancer by using 5-aminolevulinic acid. Int J Cancer 125, 2256-63 (2009).

11. Intracellular dynamics of topoisomerase l inhibitor, CPT-11, by slit-scanning confocal Raman microscopy. Histochem Cell Biol 132, 39-46 (2009).

12. Label-free biochemical imaging of heart tissue with high-speed sointaneous Raman microscopy. Biochem. Biophys Res Commun 382, 370-374 (2009).

13. Burst Emergence of Intracellular Ca2+ Waves Evokes Arrhythmogenic
Oscillatory Depolarization via the Na+-Ca2+ Exchanger Simultaneous Confocal Recording of Membrane Potential and Intracellular Ca2+ in the Heart. Circ Res 103, 509-18 (2008).

14. Generation of reentrant arrhythmias by dominant-negative inhibition of
connexin43 in rat cultured myocyte monolayers. Cardiovasc Res 79, 70-9

15. In situ Ca2+ dynamics of Purkinje fibers and its interconnection with subjacent ventricular myocytes. J Mol Cell Cardiol 38, 561-9 (2005).

16. Excitation-dependent Intracellular Ca2+ waves at the border zone of the
cryo-injured rat heart revealed by real-time confocal microscopy. J Mol Cell
Cardiol 34, 1501-1512 (2002).

17. Three distinct types of Ca2+ waves in Langendorff-perfused rat heart revealed by real-time confocal microscopy. Circ Res 86, 1093-1099 (2000).


tel 81-75-251-5322
fax 81-75-251-5353
e-mail hideotan@koto.kpu-m.ac.jp

602-8566 Kyoto-shi, Kamigyo-ku Kajii-cho,
Kawaramachi-Hirokoji, JAPAN